Search results for "differential expression"

Retrotranspozonu struktūra parastās priedes (Pinus sylvestris L.) genomā un to ekspresija

2014

ANOTĀCIJA Promocijas darbs izstrādāts Latvijas Valsts mežzinātnes institūtā „Silava”. Pētījuma mērķis bija identificēt aktīvos retrotranspozonus parastās priedes (Pinus sylvestris L.) genomā un raksturot to transkripcijas līmeni dažādu stresa veidu ietekmē kontrolētos apstākļos. Pētījumā izmantoti nespecifiskie retrotranspozonu marķieri balstīti uz augsti konservatīvām tRNS piesaistes vietām. Viena klona priežu rametus pakļāva dabā izplatītam karstuma stresam, kukaiņu invāzijai (priežu hermess (Pineus pini L.), kā arī mākslīgi izraisītai augu hormonu (abscīzskābes un salicilskābes) ietekmei. Darba rezultātā identificēti pārsvarā I klases mobīlo elementu pārstāvji, kā arī tos saturošie himēr…

Identification and characterization of durum wheat microRNAs in leaf and root tissues.

2017

MicroRNAs are a class of post-transcriptional regulators of plant developmental and physiological processes and responses to environmental stresses. Here, we present the study regarding the annotation and characterization of MIR genes conducted in durum wheat. We characterized the miRNAome of leaf and root tissues at tillering stage under two environmental conditions: irrigated with 100% (control) and 55% of evapotranspiration (early water stress). In total, 90 microRNAs were identified, of which 32 were classified as putative novel and species-specific miRNAs. In addition, seven microRNA homeologous groups were identified in each of the two genomes of the tetraploid durum wheat. Differenti…

A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

2018

The endometrium undergoes extensive changes to prepare for embryo implantation and microRNAs (miRNAs) have been described as playing a significant role in the regulation of endometrial receptivity. However, there is no consensus about the miRNAs involved in mid-secretory endometrial functions. We analysed the complete endometrial miRNome from early secretory (pre-receptive) and mid-secretory (receptive) phases from fertile women and from patients with recurrent implantation failure (RIF) to reveal differentially expressed (DE) miRNAs in the mid-secretory endometrium. Furthermore, we investigated whether the overall changes during early to mid-secretory phase transition and with RIF conditio…

Working with (too) Few Samples

2018

This paper is concerned with gene set differential expression analysis. We compare the transcriptomic behaviour of each gene set between different experimental conditions.The gene set is previously defined. It has been used a gene set collection downloaded from Gene Ontology.A randomization test is proposed and compared with other previous procedures using a RNA-seq experiment of colorectal cancer (CRC).

Cadmium-Associated Molecular Signatures in Cancer Cell Models

2021

Simple Summary The exposure of cancer cells to cadmium compounds may be associated with the acceleration of tumor progression. It is known that cadmium is a transcriptional regulator, and the study of differentially expressed genes has enabled the identification and classification of cadmium-associated molecular signatures as useful biomarkers that are potentially transferable to clinical research. This review recapitulates the studies that report the detection of such signatures in breast, gastric, colon, liver, lung, and nasopharyngeal tumor cell models, as specifically demonstrated by individual gene or whole genome expression profiling. Abstract The exposure of cancer cells to cadmium a…

Circulating microRNA biomarkers for metastatic disease in neuroblastoma patients

2018

AbstractIn this study, the circulating miRNome from diagnostic neuroblastoma serum was assessed for identification of non-invasive biomarkers with potential in monitoring metastatic disease. After determining the circulating neuroblastoma miRNome, 743 miRNAs were screened in two independent cohorts of 131 and 54 patients. Evaluation of serum miRNA variance in a model testing for tumor stage, MYCN status, age at diagnosis and overall survival, revealed tumor stage as the most significant factor impacting miRNA abundance in neuroblastoma serum. Differential expression analysis between patients with metastatic and localized disease revealed 9 miRNAs strongly associated with metastatic stage 4 …

MicroRNAs Associated With Biological Pathways of Left- and Right-sided Colorectal Cancer.

2020

BACKGROUND/AIM MicroRNAs (miRNAs) regulate the development of colorectal cancer (CRC). We aimed to investigate miRNAs and their relation to cancer-related signaling pathways in site-specific CRC. MATERIALS AND METHODS We used a total of 24 left- and right-sided Finnish CRC samples (discovery cohort) and The Cancer Genome Atlas public mature miRSeq dataset of 201 CRC samples (validation cohort). MiRNA differential expression and biological pathway analyses were performed using DESeq2 and the DIANA/mirPath tool, respectively. RESULTS We found 17 significantly differentially up-regulated [false discovery rate (FDR) <0.05] miRNAs in left-sided CRC ("left miRNAs"), and 15 in right-sided CRC ("ri…

Differential expression of tumorspheres in CSC-markers and signaling pathways from non-small cell lung cancer.

2016

e23276Background: Chemoresistance, tumor progression and metastasis have made of lung cancer the first cause of death cancer-related worldwide. Cancer stem cells (CSCs) are small subpopulations of ...

231 DIFFERENTIAL EXPRESSION PATTERNS OF CYTOKINES IN CRPS I

2007

Differential expression of the agr locus of Listeria monocytogenes EGD-e

2007

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